Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic index for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.

Tribioscience’s Acid Phosphatase Colorimetric Assay Kit provides a high-sensitive, simple, and direct assay approach to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect as low as 20 µU acid phosphatase activity in samples.

Direct Assays: Acid phosphatase in serum, plasma, urine, and other bio-samples.

• Flexible: Suitable for colorimetric assay.
• Accurate: Use 50 µL samples. Detection ranges from 0.4-200 µU in a 96-well plate for colorimetric assay.
• Simple and high-throughput: Just load-incubate-Read. The kit can be used for a robust method.
• Time-saving: less than 30 minutes.

1. ATP Colorimetric/Fluorometric Assay (TBS2010)
2. ADP Colorimetric/Fluorometric Assay Kit (TBS2020)
3. Glucose Oxidase Colorimetric/Fluorometric Assay (TBS2088)
4. β-Hexosaminidase Activity Assay (TBS2105)
5. Cytochrome C Oxidase Activity Assay (TBS2115)

Additional information

TBS2075_AP_Col_assay_v01_2023

TribioScience 是一家私人控股的生物技术公司，于 2010 年创立。我们的使命是让科学家在实验室的工作更轻松、更省时。我们专注于为分子生物学、生物化学、免疫学、传染病、基因治疗、神经科学、动物实验和药物开发领域的生命科学研究实验室和生物技术公司提供高质量的产品。我们的团队成员拥有超过 50 年的综合经验，我们还作为 CRO（研究合同组织）为生物技术行业和学术界提供服务。我们的动物建模服务已被用于 高排名大学的动物模型开发和体内评估。

TribioScience is a privately held biotech founded in 2010 by a group of scientists at Stanford University. Our mission is to make scientists’ jobs in the laboratory easier and less time-consuming.  We focus on offering high-quality products for life science research laboratories and biotech companies in the areas of molecular biology, biochemistry, immunology, infectious disease, gene therapy, neuroscience, animal experiment, and drug development.