The Bovine QPCR Kit is designed for the target-DNA specific detection of bovine matter in food and animal feed ingredients. The assay uses a real-time PCR-based protocol with fluoresce-probe to detect target DNA. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for bovine (Fig.1-2, and Table 1). No cross reactivity is observed with other animal species (See Table 2). The detection of target DNA confirms ingredient authenticity or prevents food fraud, ethical issues, or health concerns.

• Highly sensitive and specificity for bovine derived DNA.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: just add DNA template and water.
• No cross reactivity with other species

The kit is shipped on ice, and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

1. Set up PCR reaction for each sample in 25 µL

Internal control should be included as below: Positive Control (5 µL /reaction), or Negative Control (5 µL/reaction)

2. Suggested PCR conditions

Fig. 1: Bovine qPCR Amplification

Fig.2: DNA Concentration and qPCR amplification

Table1. Bovine qPCR Sensitivity Data

Table2: Cross-reactivity Survey

1. TBS6008: Fast Genomic DNA Extraction
2. TBS6025: Microbial DNA Magnetic Extraction
3. TBS42026: O157H7 E. Coli qPCR
4. TBS42027: STEC qPCR
5. TBS42028: Salmonella qPCR

Additional information

Data Sheet:

TBS42001_Bovine_qPCR kit_V03_2022

MSDS:

TBS42001_CATTLE_QPCR_MSDS 2022

1. 5 out of 5

   jchong – August 31, 2022

   Works pretty well, even in low concentrations the kit had clear, strong amplification curves. There were no errors with any parts of the kit.

TribioScience 是一家私人控股的生物技术公司，于 2010 年创立。我们的使命是让科学家在实验室的工作更轻松、更省时。我们专注于为分子生物学、生物化学、免疫学、传染病、基因治疗、神经科学、动物实验和药物开发领域的生命科学研究实验室和生物技术公司提供高质量的产品。我们的团队成员拥有超过 50 年的综合经验，我们还作为 CRO（研究合同组织）为生物技术行业和学术界提供服务。我们的动物建模服务已被用于 高排名大学的动物模型开发和体内评估。

TribioScience is a privately held biotech founded in 2010 by a group of scientists at Stanford University. Our mission is to make scientists’ jobs in the laboratory easier and less time-consuming.  We focus on offering high-quality products for life science research laboratories and biotech companies in the areas of molecular biology, biochemistry, immunology, infectious disease, gene therapy, neuroscience, animal experiment, and drug development.