CV-HLVD-LCV Multiplex RT-PCR Detection Kit is used for the detection of cryptic virus (CV), hop latent viroid(HLVD), and lettuce chlorosis (LCV) from the cannabis plant in one step RT-PCR reaction. It combines both reverse transcription (RT) and Taqman probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy-to-use, and reliable alternative to conventional “two-step” sequential qRT-PCR for detecting CV, HLVd, and LCV from plant samples.

Tribioscience Multiplex RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer probes, internal control, positive control, negative control, and buffer. The 3 target genes are labeled with a specific fluorescence color: Cy5 for Cryptic virus, Fam for HLVD, ROX for lettuce chlorosis virus, and Hex for internal control.

• This kit is used for the detection of CV, HLVd, and LCV infection in plant samples.

• One-step complete qRT-PCR in a single tube.
• Reduce contamination in the operating process.
• Accurate detection and quantification of HLVd target gene.
• 4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.

Store all components at -20°C in a non-frost-free freezer. Shelf life is 12 months after receipt. The kit is shipped on ice.

1. TBS6025: Microbial DNA Magnetic Extraction
2. TBS42018: Trichothecene-producing Fusarium Species TaqProbe qPCR Detection
3. TBS42015: HLVD RT-qPCR detection
4. TBS42019: Fusarium Species qPCR Detection
5. TBS42020: Universal Aspergillus qPCR

Additional information

TBS42016_Taqman_qRT-PCR_CV-HLVd-LCV_V01_2023

TribioScience 是一家私人控股的生物技术公司，于 2010 年创立。我们的使命是让科学家在实验室的工作更轻松、更省时。我们专注于为分子生物学、生物化学、免疫学、传染病、基因治疗、神经科学、动物实验和药物开发领域的生命科学研究实验室和生物技术公司提供高质量的产品。我们的团队成员拥有超过 50 年的综合经验，我们还作为 CRO（研究合同组织）为生物技术行业和学术界提供服务。我们的动物建模服务已被用于 高排名大学的动物模型开发和体内评估。

TribioScience is a privately held biotech founded in 2010 by a group of scientists at Stanford University. Our mission is to make scientists’ jobs in the laboratory easier and less time-consuming.  We focus on offering high-quality products for life science research laboratories and biotech companies in the areas of molecular biology, biochemistry, immunology, infectious disease, gene therapy, neuroscience, animal experiment, and drug development.