Retro-CAG-eGFP-VSVG was packaged in HEK293T cells with Ca₃(PO₄)₂ transfection of 3 plasmids of pRetro-CAGGFP, pRetro-RC, and pCMV-VSVG. The pRetro-CAGGFP was constructed by using the components of Moloney Murine Leukemia virus (MoMLV), a combination of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter (CAG), and enhanced green fluorescent protein (eGFP) expression cassette. RetroCAG-eGFP-VSVG is a replication deficient virus and has potentials to infect many types of cell and tissue. It belongs to biosafety level-1 (BSL-1). Retro-CAG-eGFP-VSVG is provided as concentrated viral supernatant at a titer around 107 IU/ml in HEK293T cells.

Feature :

• Bright GFP expression: The Retro-CAG-eGFP-VSVG can express the green fluorescent protein brightly in many cultured cells and animals.
• Replication deficient virus: The Retro-CAG-eGFPVSVG is replication deficient virus.

Applications :

• Virology: The Retro-CAG-eGFP-VSVG can be used as a control virus in virological researches.
• Gene therapy research: The Retro-CAG-eGFP-VSVG can be used as a control retroviral vector when the targeted gene expressed by using retroviral vector.
• Cell biology: The Retro-CAG-eGFP-VSVG can be used for establishing stable GFP expression cell lines.

Storage Conditions :

• The product is shipped on dry ice. Store at -80°C.

Additional information

TBS7201_Retro-CAG-eGFP-VSVG

TribioScience 是一家私人控股的生物技术公司，于 2010 年创立。我们的使命是让科学家在实验室的工作更轻松、更省时。我们专注于为分子生物学、生物化学、免疫学、传染病、基因治疗、神经科学、动物实验和药物开发领域的生命科学研究实验室和生物技术公司提供高质量的产品。我们的团队成员拥有超过 50 年的综合经验，我们还作为 CRO（研究合同组织）为生物技术行业和学术界提供服务。我们的动物建模服务已被用于 高排名大学的动物模型开发和体内评估。

TribioScience is a privately held biotech founded in 2010 by a group of scientists at Stanford University. Our mission is to make scientists’ jobs in the laboratory easier and less time-consuming.  We focus on offering high-quality products for life science research laboratories and biotech companies in the areas of molecular biology, biochemistry, immunology, infectious disease, gene therapy, neuroscience, animal experiment, and drug development.